The A.E. "Bean" Backus Museum celebrates the Florida landscape painter and the Highwaymen artists he mentored.
The African American Museum of the Arts in DeLand shows art and artifacts of African, African American and Caribbean cultures.

The African American Research Library and Cultural Center is a Broward County library focused on the African diaspora.
The Ah-Tah-Thi-Ki Museum on the Big Cypress Seminole Indian Reservation is the Seminole Tribe of Florida's museum.
The Air Force Armament Museum outside Eglin Air Force Base displays aircraft, missiles and weapons history.

The Headquarters Library in downtown Gainesville is the main branch of the Alachua County Library District.
The Amelia Island Museum of History is in Nassau County's former 1935 jail.
The SS American Victory is a World War II cargo ship preserved as a museum in Tampa.
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